Death of protoplasm is a prerequisite for a vital function like
transport of sap
transport of food
absorption of water
gaseous exchange.
Death of protoplasm is a prerequisite for a vital function like transport of sap. Xylem is a dead tissue (except xylem parenchyma) and does not have protoplasm, xylem performs the function of transport of water or sap inside the plant from roots to leaves. Hence, the correct answer is option 1.