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  • During the preparation of recombinant DNA, which of the following enzymes is used to join the cut-out 'gene of interest' from the source DNA and the cut vector with space?

During the preparation of recombinant DNA, which of the following enzymes is used to join the cut-out 'gene of interest' from the source DNA and the cut vector with space?

Option: 1

 Restriction enzyme


Option: 2

 Polymerase chain reaction


Option: 3

 Ligase

 


Option: 4

Reverse transcriptase


Answers (1)

best_answer

 During the preparation of recombinant DNA, after cutting the source DNA and vector DNA with a specific restriction enzyme, the cut-out 'gene of interest' from the source DNA and the cut vector with space are mixed and ligase is added. Ligase catalyzes the formation of phosphodiester bonds between the sugar and phosphate groups of adjacent nucleotides, thereby joining the cut DNA fragments and forming recombinant DNA

Option 3 is the correct answer.

 

Posted by

Kshitij

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