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ELISA assay uses
An enzyme which can react with secondary antibodies.
An enzyme which can react with the antigen.
A substrate which gets converted into a colored product.
A adio labeled secondary antibody.
Answers (1)
ELISA (Enzyme-Linked Immunosorbent Assay) is a commonly used biochemical assay that utilizes a substrate that can be converted into a colored product by an enzymatic reaction.
In ELISA, the substrate is usually a chromogenic compound that is linked to an enzyme, such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). These enzymes catalyze specific reactions that result in the conversion of the substrate into a colored or fluorescent product.
The color or fluorescence produced is directly proportional to the amount of the target analyte (e.g., antigen or antibody) present in the sample being tested. The color intensity or fluorescence signal is then measured using a spectrophotometer or a specialized reader, allowing for the quantification or detection of the analyte.
The enzymatic reaction in ELISA is typically triggered by the addition of a substrate solution that contains the substrate for the enzyme conjugate. If the target analyte is present in the sample, it binds to the capture or detection antibodies immobilized on the ELISA plate. Subsequent incubation with the enzyme-conjugated secondary antibody and the substrate solution leads to the enzymatic conversion of the substrate into a colored or fluorescent product. The color or fluorescence intensity is then measured and correlates with the concentration of the target analyte in the sample.
The use of a substrate that generates a colored product is a key feature of ELISA, as it allows for visual detection or quantitative measurement of the target analyte, making it a widely employed technique in various fields, including clinical diagnostics, research, and quality control.
Hence, the correct answer is option 3.
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