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  • Give answer! Commonlyused vectors for a human genome sequencing are:

Commonly used vectors for a human genome sequencing are:

  • Option 1)

    T-DNA

  • Option 2)

    BAC and YAC

  • Option 3)

    Expression Vectors

  • Option 4)

    kT/A Cloning Vectors

 

Answers (1)

best_answer

As learnt in

Size of insert in other vectors -

Bactripohage-can take up to N 25 Kbps. 

Cosmid-constructed vector contains cos site from \lambda phage DNA with plasmid and can take up to 45-48 kb insert. 

Plasmid-Contains phase DNA and plasmid DNA and can take up to 100 kb. 

BAC-can take up to 250-300kb  insert 

YAC-can take up to 500 Kbs insert 

MAC-can take up to 1 Mbp insert 

-

 

 and

Human genome project -

This project was proposed in USA (1986). USA, Germany, France, Japan, Australia, Italy, Korea, Russia, Nederland & Sweden countries were Involved. 

- wherein

  • Human genome Contains 3164.7 million nucleotides
  • Total genes are approximately 30,000.
  • Only 2 % genome codes for protein

 

 BAC and YAC can take large kb inserts and were used in Human Genome Project.


Option 1)

T-DNA

This option is incorrect

Option 2)

BAC and YAC

This option is correct

Option 3)

Expression Vectors

This option is incorrect

Option 4)

kT/A Cloning Vectors

This option is incorrect

Posted by

prateek

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