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In the context of recombinant colonies and the X-gal compound, consider the following statements:
Assertion: Recombinant colonies will exhibit a blue color in the medium containing ‘X-gal’.
Reason: The 'gal gene' of the vector has undergone insertional inactivation.
Both the assertion and the reason are true, and the reason is the correct explanation of the assertion.
Both the assertion and the reason are true, but the reason is not the correct explanation of the assertion.
The assertion is true, but the reason is false.
The assertion is false, but the reason is true.
Answers (1)
Recombinant colonies will not necessarily exhibit a blue color in the medium containing 'X-gal.' The reason is true, stating that the 'gal gene' of the vector has undergone insertional inactivation. When foreign DNA is inserted into the 'gal gene' of the vector, it can disrupt or inactivate the gene, preventing the production of an active β-galactosidase enzyme. Without active β-galactosidase, X-gal cannot be cleaved, and therefore, the colonies will not exhibit a blue color. Hence, the correct answer is option 4.
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