Browse by Stream
QnA
Home
QnA
Go To Your Study Dashboard

Get Answers to all your Questions

header-bg qa
  • Home
  • Medical
  • In the process of distinguishing transformants from nontransformants, which factor/s contributes to the selection of transformants?Opt

In the process of distinguishing transformants from nontransformants, which factor/s contributes to the selection of transformants?

Option: 1

 Existence of multiple recognition sites in the vector DNA.


Option: 2

Insertional inactivation of the selectable marker due to the incorporation of foreign DNA.


Option: 3

Inactivation of the antibiotic resistance gene caused by the insertion of foreign DNA.


Option: 4

 Both (b) and (c) play a role in the selection process.


Answers (1)

best_answer

In the process of selecting transformants from nontransformants, both option (2) and option (3) are correct and contribute to the selection process.

Option (2) states that the presence of alien DNA in the vector DNA results in the insertional inactivation of the selectable marker. This means that when foreign DNA is successfully incorporated into the vector, it disrupts the functioning of the selectable marker, leading to the loss of its activity in nontransformants. Transformants, on the other hand, retain the selectable marker activity.

Option (3) states that the antibiotic resistance gene gets inactivated due to the insertion of alien DNA. Similar to option (2), when foreign DNA is inserted into the vector, it disrupts the functioning of the antibiotic resistance gene. As a result, nontransformants lose their resistance to the antibiotic, while transformants, which carry the foreign DNA, retain their resistance.

Both options (2) and (3) are valid mechanisms for distinguishing transformants from nontransformants in the context of recombinant DNA technolo. Hence, the correct answers option 4.

Posted by

Ritika Harsh

View full answer

NEET 2024 Most scoring concepts

    Just Study 32% of the NEET syllabus and Score up to 100% marks

Similar Questions

Trending Articles/News

anam-khan

NEET UG 2025: Top government medical colleges with 5 years’ cut-off ranks; AIIMS Delhi most sought-after
anam-khan

NEET UG 2025 information bulletin soon; optional questions removed, exam in OMR pen-paper mode
anam-khan

Aiming for top medical colleges in India? Rank-wise NEET UG cut-offs for MBBS courses

Latest Question