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  • In the process of inserting a human gene into bacteria, a scientist mistakenly uses a different restriction enzyme on the human gene compared to the one used on the plasm

In the process of inserting a human gene into bacteria, a scientist mistakenly uses a different restriction enzyme on the human gene compared to the one used on the plasmid. Predict the most probable outcome of this error:

Please select the option that best represents the anticipated consequence of the error

Option: 1

The restriction enzyme will fail to cleave the human gene.


Option: 2

The bacterial DNA will remain intact without being cleaved by the restriction enzyme. 


Option: 3

The plasmid will not successfully integrate the human gene.

 


Option: 4

The bacteria will be incapable of hosting the plasmid.


Answers (1)

best_answer
  • When different restriction enzymes are used on the human gene and the plasmid, it means that the recognition sites for cutting the DNA will not match. Restriction enzymes recognize specific DNA sequences and cleave the DNA at those sites. If the scientist uses different enzymes, the cuts made on the human gene and the plasmid will not be compatible.
  • As a result, when attempting to insert the human gene into the plasmid, the incompatible ends will not properly align and join together. This will hinder the successful incorporation of the human gene into the plasmid.
  • Options A and B are incorrect because restriction enzymes are still capable of cutting DNA, although at different locations. The enzyme used on the human gene will cut it at its specific recognition site, and the enzyme used on the plasmid will cut it at its respective recognition site.
  • Option D is also incorrect because the ability of bacteria to accommodate the plasmid is not directly affected by the use of different restriction enzymes. The primary issue lies in the mismatched DNA ends preventing successful gene insertion into the plasmid.
  • Therefore, the most probable outcome in this scenario is that the plasmid will not incorporate the human gene.
  • Hence, the correct answer is option 3.
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