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Match the following,  Column I containing names of the vector with their respective DNA fragment size present in Column II.

  Vector   DNA fragment size
1. Plasmids (pBR233,PUC19) A. 3000kb
2. Bacteriophage (M13 phage, \lambda- phage) } B. 28-45kb
3. Cosmids C. 350kb
4. BACs (Bacterial Artificial Chromosomes) D. 15kb
5. YACs (Yeast Artificial Chromosomes) E. 53kb

 

Option: 1

1.D , 2.E, 3.B, 4.C, 5.A

 


Option: 2

1.E , 2.D, 3.B, 4.C, 5.A


Option: 3

1.C , 2.E, 3.A, 4.D, 5.B


Option: 4

1.B , 2.E, 3.D, 4.A, 5.C


Answers (1)

Commonly used cloning vectors and their capacity to clone DNA fragments of size is given below. 

Vector DNA fragment size
Plasmids (pBR233,PUC19) 15 kb
Bacteriophage (M13 phage, \lambda-phage) 53 kb
Cosmids 28-45 kb
BACs (Bacterial Artificial Chromosomes) 350 kb
YACs (Yeast Artificial Chromosomes) 3000 kb

Hence, the correct answer is option 1.

Posted by

Sumit Saini

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