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  • Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance.If this enzymes is used for inserting a gene <img alt="\beta" src="/latex-im

Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance.If this enzymes is used for inserting a gene \beta- galactoside production and the recombinant plasmid is inserted in an E.coli strain 

Option: 1

it will not be able to confer ampicilin resistance to the host cell


Option: 2

the transformed cells will have the ability to resist ampicillin as well as produces \beta - galactoside


Option: 3

it will lead to lysis of host cell


Option: 4

it will be able to produce a novel protein with dual ability


Answers (1)

best_answer

Since the gene is inserted at Pst I of ampR region of pBR322, the ampR gene is inactivated which is called as insertional inactivation. Hence the genetically modified E. coli strain will not be able to confer ampicillin resistance.

Posted by

Deependra Verma

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