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Read the following statements and select the correct option w.r.t insertional inactivation
Statement 1: Presence of insert results into insertional inactivation of the â-galactosidase and the colonies do not produce any color, these are identified as recombinant colonies.
Statement 2: The presence of a chromogenic substrate gives blue colored colonies if the plasmid in the bacteria does not have an insert.
Option: 1
Statement 1 is correct but statement 2 is incorrect.
Option: 2
Statement 1 is incorrect but statement 2 is correct
Option: 3
Both statements are incorrect
Option: 4
Both statements are correct.
Answers (1)
As we have already studied in pBR322 Plasmid Vector & Mode of Selection of Transformants -
- A recombinant DNA is inserted within the coding sequence of an enzyme, â-galactosidase.
- This results in the inactivation of the enzyme, which is referred to as insertional inactivation.
- The presence of a chromogenic substrate gives blue colored colonies if the plasmid in the bacteria does not have an insert.
- Presence of insert results into insertional inactivation of the â-galactosidase and the colonies do not produce any color, these are identified as recombinant colonies.
- Hence, the correct option is (d).
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