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Reverse transcriptase has both ribonuclease and polymerase activities. Ribonuclease activity is required for
the synthesis of new RNA strand
the degradation of RNA strand
the synthesis of new DNA strand
the degradation of DNA strand
Answers (1)
The ribonuclease activity of reverse transcriptase is required for the degradation of the RNA strand during reverse transcription.
Reverse transcriptase is an enzyme that catalyzes the synthesis of complementary DNA (cDNA) from an RNA template. It possesses both polymerase activity, which allows it to synthesize DNA, and ribonuclease activity, which allows it to degrade RNA.
During reverse transcription, reverse transcriptase uses the RNA template to synthesize a complementary DNA strand. However, in order to generate a complete cDNA molecule, the RNA template must be removed. This is achieved through the ribonuclease activity of reverse transcriptase.
The ribonuclease activity of reverse transcriptase degrades the RNA template strand, breaking it down into individual ribonucleotides. This degradation of the RNA strand leaves behind a single-stranded cDNA molecule.
Once the RNA template is degraded, the polymerase activity of reverse transcriptase takes over. It uses the degraded RNA strand as a primer and adds deoxyribonucleotides to synthesize the complementary DNA strand. This results in the synthesis of a double-stranded cDNA molecule.
In summary, the ribonuclease activity of reverse transcriptase is required for the degradation of the RNA strand during reverse transcription, enabling the synthesis of complementary DNA from an RNA template
Hence, the correct answer is option 2.
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