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Statement I - In blue/white colonies selection, recombinants produce blue colonies due to insertional inactivation of β-galactosidase.
Statement II - The transformants produced during genetic engineering include both recombinants and non-recombinants.
Option: 1
Both statements are correct.
Option: 2
Only statement I is incorrect.
Option: 3
Only statement II is incorrect.
Option: 4
Both statements are incorrect.
Answers (1)
- Statement I is incorrect because in blue/white colony selection, recombinants produce white colonies, not blue colonies. The inactivation of β-galactosidase by the inserted DNA leads to the accumulation of a colorless substrate called X-gal, resulting in the white colonies. Non-recombinants, on the other hand, produce active β-galactosidase, which hydrolyzes X-gal and produces a blue color.
- Statement II is correct because the transformants produced during genetic engineering can include both recombinants (cells that have undergone the desired genetic modification) and non-recombinants (cells that have not undergone the desired genetic modification). This is because not all cells in the transformation process may take up and integrate the foreign DNA, leading to a mixture of transformed and non-transformed cells.
- Hence, the correct answer is option (2) which is Only statement I is incorrect.
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