The dnaB gene of E.coli encodes a helicase (DnaB) that unwinds DNA at the replication fork. Select the correct statement about unwinding of DNA.
Unwinding of DNA is an active process.
Helicase enzyme binds to the template of lagging strand and moves along it in 3’ to 5’ direction.
The separated strands are inhibited from subsequently reannealing by DNA polymerases, which bind to both separated strands.
All of the above.
Helicase enzyme binds to the template of lagging strand and moves along it in 5’ to 3’ direction.The direction is always defined according to the strand of DNA bound. The separated strands are inhibited from subsequently reannealing by a single-strand binding protein (SSB), which binds to both separated strands. SSB protein is a tetramer and binds cooperatively in a sequence-independent manner. Hence, the correct answer is option 1.