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The ‘heat shock method’ in bacterial transformation is used to facilitate
Option: 1
DNA binding to the cell wall
Option: 2
DNA uptake through membrane transport proteins
Option: 3
DNA uptake through transient pores present in the bacterial cell wall
Option: 4
The expression of antibiotic-resistant genes
Answers (1)
As we have already studied in Competent Host (For Transformation with Recombinant DNA) -
In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall. Hence, the correct option is (c).
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