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  • The nuclease enzyme which begins its attack from a free end of a polynucleotide is:  Option: 1 Exonuclease<div class='qn

The nuclease enzyme which begins its attack from a free end of a polynucleotide is:

 

Option: 1

Exonuclease


Option: 2

Kinase


Option: 3

Endonuclease


Option: 4

Polymerase


Answers (1)

best_answer

Exonuclease activity means cleavage of nucleotides only at the end while endonuclease breaks DNA strand at an internal position. DNA polymerase I has 3' - 5' exonuclease activity which removes any nucleotide which mispair during elongation of growing strand. A small segment of DNA polymerase I also shows 5' - 3' exonuclease activity which removes the DNA segment which comes as an obstruction in the way of growing DNA strand. Polymerase catalyzes the elongation of a polymeric molecule. Endonucleases are very specific and cut DNA at very specific nucleotide sequences. These are called restriction enzymes. Kinase is an enzyme that can transfer a phosphate group.

Hence, the correct answer is option 1. 

Posted by

Kuldeep Maurya

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