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The process of "overloading" in column chromatography refers to:
Option: 1
Using too much stationary phase
Option: 2
Using too much mobile phase
Option: 3
Applying excessive pressure to the column
Option: 4
Loading an excessive amount of sample on the column
Answers (1)
The process of "overloading" in column chromatography refers to loading an excessive amount of sample onto the column, which can lead to poor separation and band broadening.
Hence option 4 is correct.
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