To detect mutation (GAG -> GTG) allele specific hybridization method is used. Four members of an affected family are investigated. DNA isolated from blood samples of parents and two offsprings are spotted on a membrane after ('3 appropriate processing and probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTOTGGAGAAGTE (second probe) after labelling. While probed with first oligonucleotide, signals are obtained for the positions where DNA is spotted from parents and offspring II. When probed with a second oligonucleotide, signals are obtained at the position where DNA from the parents and offspring I spotted. Results are shown below:
Father | Mother | Offspring I | Offspring II | |
First probe | + | + | - | + |
Second probe | + | + | + | - |
On the basis of the result, which of the following statements is correct?
Parents are affected
Offspring I is affected
Offspring Il is carrier
Offspring Il is affected
Only offspring I is affected among all. Hence, the correct answer is option 2.