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  • Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as adaptation. These i

Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as adaptation. These imprints need to be used

 

Option: 1

on plates with and without streptomycin


Option: 2

on plates with minimal medium


Option: 3

only on plates with streptomycin


Option: 4

ónly on plates without streptomycin.


Answers (1)

best_answer

Streptomycin is broad spectrum (active against both Gram-positive and Gram-negative bacteria) and was the first really effective drug against tuberculosis, but its use is limited by the development of resistant strains and by toxic side-effects. The bactericidal action of streptomycin, as with other aminoglycoside antibiotics (e.g., neomycin) is through selective inhibition of protein synthesis on 705 ribosomes. To check resistance of mutants against streptomycin they must be grown on plates with streptomycin. Only those bacterial colonies will propagate from the master that is resistant to the antibiotic.

Hence, the correct answer is option 3.

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mansi

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