What conclusions can be drawn from the depicted graph illustrating the relationship between substrate concentration and the reaction rate of green-gram-phosphatase enzyme?
The rate of enzymatic reaction exhibits a positive correlation with the concentration of the substrate.
The reaction mixture contains an inhibitor that affects the enzyme.
The graph suggests the formation of an enzyme-substrate complex.
Higher substrate concentrations lead to an elevation in pH levels.
The graph indicates that higher substrate concentrations result in an increase in pH levels. This suggests that the green-gram-phosphatase enzyme's activity is influenced by the pH of its environment. The enzyme may function optimally in an alkaline pH range, leading to an enhanced reaction rate at higher substrate concentrations. Thus, the graph implies a correlation between substrate concentration, pH elevation and the enzyme's activity.
Hence, the correct answer is option 4.