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What is the error in the procedure given below, which outlines the basic steps involved in synthesizing a recombinant DNA molecule?
Polymerase enzyme is not utilized in the process.
The mammalian DNA shown is having a double-stranded structure.
A single fragment is inserted.
Two different restriction enzymes are utilized.
Answers (1)
In the basic steps involved in synthesizing a recombinant DNA molecule, if the statement mentions that only one fragment is inserted, it could be an incomplete or inaccurate step. In most cases, multiple DNA fragments are inserted to create a recombinant DNA molecule. The number of fragments inserted depends on the experiment's design and desired outcome. It is essential to consider the possibility of incorporating additional fragments or genetic elements to achieve the desired result. Hence, the correct answer is option 3.
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