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  • What is the optimal order of procedures for integrating foreign DNA into a plasmid and introducing the plasmid into a bacterium? Introducing the rDNA mo

What is the optimal order of procedures for integrating foreign DNA into a plasmid and introducing the plasmid into a bacterium?

  1. Introducing the rDNA molecule into bacteria
  2. Including cutting the plasmid DNA with restriction enzymes
  3. Extracting plasmid DNA from bacterial cells
  4. H-bonding the plasmid DNA to non-plasmid DNA fragments
  5. Utilizing ligase to join the plasmid DNA with non-plasmid DNA
Option: 1

1,2,4,3,5


Option: 2

2,3,5,4,1


Option: 3

3,2,4,5,1


Option: 4

3,4,5,1,2


Answers (1)

best_answer

The optimal order of procedures for integrating foreign DNA into a plasmid and introducing the plasmid into a bacterium is as follows:

3. Extract plasmid DNA from bacterial cells.

2. Including cutting the plasmid DNA with restriction enzymes

4. Introduce the rDNA molecule into bacteria.

5. Utilizing ligase to join the plasmid DNA with non-plasmid DN

1. Introducing the rDNA molecule into bacteria

Hence, the correct answer is option 3.

Posted by

shivangi.bhatnagar

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