Which of the following enzymes is responsible for removing RNA primers and filling in the gaps with DNA nucleotides during DNA replication?
DNA polymerase I
DNA polymerase III
DNA helicase
DNA ligase
DNA polymerase III is responsible for synthesizing new DNA strands during DNA replication. It has a 5' to 3' exonuclease activity that allows it to remove RNA primers and fill in the gaps with DNA nucleotides. DNA polymerase I is also involved in this process but primarily in removing the RNA primers. DNA helicase is responsible for unwinding the double-stranded DNA, and DNA ligase is responsible for joining the Okazaki fragments on the lagging strand.
Option 2 is the correct answer.