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  • Which of the following is correct sequence of steps in PCR (Polymearse Chain Reaction)?Option: 1 Denaturation, Annealing , Extenstion<d

Which of the following is correct sequence of steps in PCR (Polymearse Chain Reaction)?

Option: 1

Denaturation, Annealing , Extenstion


Option: 2

Denaturation, Extension, Annealing


Option: 3

Extension, Denaturation, Annealing


Option: 4

Annealing, Denaturation, Extension


Answers (1)

PCR is based on three simple steps required for any DNA synthesis reaction:

(1) denaturation of the template into single strands;

(2) annealing of primers to each original strand for new strand synthesis; and

(3) extension of the new DNA strands from the primers.

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Kshitij

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