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Which of the following is correct sequence of steps in PCR (Polymearse Chain Reaction)?
Option: 1
Denaturation, Annealing , Extenstion
Option: 2
Denaturation, Extension, Annealing
Option: 3
Extension, Denaturation, Annealing
Option: 4
Annealing, Denaturation, Extension
Answers (1)
PCR is based on three simple steps required for any DNA synthesis reaction:
(1) denaturation of the template into single strands;
(2) annealing of primers to each original strand for new strand synthesis; and
(3) extension of the new DNA strands from the primers.
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