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- #Biotechnology and Its Applications
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Which of the following is required to perform polymerase chain reaction ?
Option: 1
Primers , dNTPs and DNA polymerase
Option: 2
DNA, CaCl2 and nuclease
Option: 3
Mg+2, DNA
Option: 4
Both a and c
Answers (1)
As we have learned
Extension -
(Extension= Polymerization). This is final step in which Taq DNA polymerase recognised - 3' free-OH group on primer. Taq polymerase adds complementary nucleotides in the presence of (as a cofactor).
- wherein
This step is carried out at
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