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- #Class 11
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- #Cell Structure and Function
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Which of the following is used for the chemical analysis of the living tissue?
Option: 1
Tribromoacetic acid
Option: 2
Tri Acetoacetic acid
Option: 3
Trichloroacetic acid
Option: 4
All of these
Answers (1)
Analysis of Chemical Composition in Living Tissues -
Analysis of Chemical Composition in Living Tissues
It can be done by the following two methods:
Chemical Analysis:
- By grinding any living tissue (like a piece of vegetable) with trichloroacetic acid (CCl3COOH) using a mortar and pestle.
- A thick slurry obtained is strained with a cheesecloth.
- Two fractions would be obtained as:
- Filtrate or acid soluble pool
- Retentate or acid insoluble fraction
- The acid soluble pool consisted of micromolecules.
- Their molecular weight is less than 1000 Da.
- Amino acids, sugars, nucleotides are micromolecules.
- The acid insoluble pool consisted of macromolecules whose molecular weight is more than 1000 Da.
- Carbohydrates, nucleic acids, proteins are macromolecules.
- The acid insoluble fraction also included lipids. However, lipids are micromolecules as they never weigh more than 800 Da. This happened because the lipid components of the plasma membrane and organelle membranes form vesicles which are insoluble. So, they appear along with acid insoluble fraction despite being a micromolecule.
Trichloroacetic acid is used for the chemical analysis of the living tissues.
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