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Which one of the following statement is not true regarding gel electrophoresis technique?
Option: 1
The separated DNA fragments are stained by using ethidium bromide.
Option: 2
The presence of chromogenic substrate gives blue coloured DNA bands on the gel.
Option: 3
Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.
Option: 4
The process of extraction of separated DNA strands from gel is called elution.
Answers (1)
In agarose gel electrophoresis, bands are visualized as orange bands with EtBr (Ethiclium Bromide). Etbr on intercalation with nitrogenous bases gives orange colored bands on gel and it shows fluorescence upon UV exposure. Protein bands stain blue on SDS-PAGE.
Hence, the correct option is (2).
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