A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:
a. A human gene may have intron which bacteria cannot process
b. Amino acid codons for humans and bacteria are different
c. Human protein is formed but degraded by bacteria
d. All of the above
The answer is option (a) Human gene may have intron which bacteria cannot process.
Explanation: Any nucleotide sequence in a gene that is removed by RNA splicing during the maturation of the final RNA product is called an intron. Intron can be different in different organisms.