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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?

a. Easy availability of DNA template
b. Availability of synthetic primers
c. Availability of cheap deoxyribonucleotides
d. Availability of 'Thermostable' DNA polymerase

Answers (1)

The answer is option (d) Availability of 'Thermostable' DNA polymerase.

Explanation: Thermostable DNA is isolated from the bacterium Thermus aquaticus. It remains active during high temperature-induced denaturation of DNA. Before the discovery of thermostable polymerase, the polymerase in use suffered denaturation during the heat treatment step of the process.

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Gurleen Kaur

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