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'Restriction' in Restriction enzyme refers to:

a. Cleaving of the phosphodiester bond in DNA by the enzyme
b. Cutting of DNA at a specific position only
c. Prevention of the multiplication of bacteriophage by the host bacteria
d. All of the above

Answers (1)

The answer is option (c) Prevention of the multiplication of bacteriophage in bacteria.

Explanation: Restriction enzymes belong to a class of enzymes called nuclease. Restriction enzymes prevent the multiplication of bacteriophage in bacteria. They are used for cutting DNA at specific positions which becomes possible by cleaving of phosphodiester bonds in DNA by the enzyme. Option 'c' is the correct answer.

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Gurleen Kaur

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