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  • The most important feature in a plasmid to serve as a vector in gene cloning experiment is a. Origin of replication (ori) b. Presence of a selectable marker

The most important feature in a plasmid to serve as a vector in gene cloning experiment is:

a. Origin of replication (ori)
b. Presence of a selectable marker
c. Presence of sites for restriction endonuclease
d. Its size

Answers (1)

The answer is the option (a) Origin of replication (ori)

Explanation: Origin of replication is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. This sequence is also responsible for controlling the copy number of the linked DNA. Therefore, if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin support high copy number.

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Gurleen Kaur

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