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  • A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

Answers (1)

The following are the possible reasons for the non-observation of DNA bands:

(a) DNA may have been contaminated because of the accidental addition of nuclease enzyme.

(b) Electrodes may have been put in the wrong orientation with the anode towards the loading well. As DNA is negatively charged, it moves towards the anode. When the anode is near the loading well, separated DNA may move out of the gel.

(c) Quantity of ethidium bromide may not have been sufficient.

(d) Exposure to UV is an important step and it's done in this situation. 

Posted by

Gurleen Kaur

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