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  • A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e. bacterial ransformation?

A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment, i.e. bacterial transformation?

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When a DNA molecule is created by ligating a gene to a plasmid vector, it becomes a circular DNA which is ready to replicate in the host organism. The addition of exonuclease is not going to affect the process after this stage because the DNA does not have a free end, and hence enzyme exonuclease will not get a substrate to show its action. Therefore, in this experiment, bacterial transformation is not going to be disturbed.

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Gurleen Kaur

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