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Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at 'specific-recognition sequence'. What would be the disadvantage if they do not cut the DNA at a specific recognition sequence?
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Specific recognition sequences in DNA provide sticky ends at which the recombination of genes takes place. This further leads to the replication of the selected gene. If endonuclease fails to cut DNA at a specific recognition sequence; then recombination or replication will fail to take place.
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