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9. What are the steps involved in formation of a root nodule?

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Formation of root nodules- The process of nodule formation involves a sequence of interaction between root and Rhizobium which occurs in the following manner-

The Rhizobia present around the roots multiply and increase in number. These Rhizobia attach themselves with the epidermis of the roots. The root hairs curls and bacteria invade it. An infection thread is formed that carries the bacteria into the cortex of root. Nodule formation starts in the cortex of root. Then, the bacteria are released from thread to cells which leads to the formation of specialized nitrogen-fixing cells. The nitrogen-fixing cells by repeated divisions increase in number and form nodules. The nodules establish a direct vascular connection with the host for the exchange of nutrients. These nodules contain all necessary biochemical components like enzyme nitrogenase and leg-haemoglobin. Due to the presence of leghaemoglobin, nodules are pinkish in colour. 

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