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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
a. Easy availability of DNA template
b. Availability of synthetic primers
c. Availability of cheap deoxyribonucleotides
d. Availability of 'Thermostable' DNA polymerase
Answers (1)
The answer is the option (d) Availability of 'Thermostable' DNA polymerase
Explanation: Thermostable DNA is isolated from a bacterium Thermus aquaticus. It remains active during high temperature induced denaturation of DNA. Before the discovery of thermostable polymerase, the polymerase in use suffered denaturation during heat treatment step of the process.
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