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  • The diffraction effects in a microscopic specimen become important when the separation between two points isOption: 1 much greater than the wavelengt

The diffraction effects in a microscopic specimen become important when the separation between two points is

Option: 1

much greater than the wavelength of light used. 


Option: 2

much less than the wavelength of light used.


Option: 3

comparable to the wavelength of light used.


Option: 4

independent of the wavelength of light used.


Answers (1)

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When the wavelength of light used is comparable with the separation between two points, the image of the object will be a f diffraction pattern whose size will be

\mathrm{\theta=\frac{1.22 \lambda}{\mathrm{D}}}

\mathrm{\text { Where } \lambda=\text { wavelength of light used }}

\mathrm{D=\text { diameter of the objective }}

Two objects whose images are closer than this distance, will not be resolved.

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