During which stage of mitosis do the centromeres split and the two chromatids of the duplicated chromosomes separate from each other?
Prophase
Metaphase
Anaphase
Telophase
During anaphase of mitosis, the centromere of each duplicated chromosome splits, and the two sister chromatids separate from each other, becoming individual daughter chromosomes. The spindle fibers attached to the kinetochores of the chromosomes pull the daughter chromosomes toward opposite poles of the cell, resulting in the separation of the duplicated genetic material. Prophase is characterized by the condensation of chromatin, formation of the spindle fibers, and disappearance of the nucleolus and nuclear membrane. Metaphase is the stage where the chromosomes align at the equatorial plane of the cell. Telophase is characterized by the formation of new nuclear envelopes around the daughter chromosomes and the division of the cytoplasm. Therefore, option 3) is the correct answer.