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Ethylene signaling pathway is important for fruit ripening. Which one of the following responses is routinely used to identify ethylene signaling pathway components?
Cotyledon expansion response
Lateral root formation response
Triple response
Flowering time response
Answers (1)
One of the routinely used responses to identify ethylene signaling pathway components is the "triple response" in etiolated seedlings. The triple response is a set of characteristic phenotypic changes observed in plant seedlings when exposed to ethylene. It includes shortening and thickening of the hypocotyl (stem-like structure below the cotyledons), exaggerated curvature of the apical hook, and inhibition of root and hypocotyl elongation.
By studying the triple response in etiolated seedlings, researchers can identify and characterize mutants or genetic variants that are either insensitive or hyper-sensitive to ethylene. These mutants help identify the components involved in the ethylene signaling pathway. For example, mutants that fail to display the triple response in the presence of ethylene can provide insights into the genes or proteins responsible for perceiving and transmitting the ethylene signal.
Therefore, the triple response in etiolated seedlings is a commonly used assay to identify and study components of the ethylene signaling pathway. Hence, the correct answer is option 3.
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