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Match the following :
|
(A) Retardation of Leaf Senescence |
(i) measure cytokinin concentration Between 0.001-10 mg/liter |
| (B) Excised Radish Cotyledon Expansion | (ii) developed by Letham |
| (C) Tobacco Pith Culture | (iii) rapid bioassay technique |
A-ii, B-i, C-iii
A-iii, B-i, C-ii
A-i, B-iii, C-ii
A-ii, B-iii, C-i
Answers (1)
1. Tobacco Pith Culture: One of two tobacco pith cultures is given cytokinin, whereas the other is not. The increase in fresh weight of the tissue above the control is a measure of cell division stimulation and thus cytokinin activity. The test can detect cytokinin concentrations ranging from 0.001 to 10 mg/liter. It takes about 3-5 weeks.
2. Leaf Senescence Retardation: This is a quick bioassay procedure. There are two lots of leaf discs. Cytokinin is delivered in a single batch. The chlorophyll content of the leaf discs is determined after 48-72 hours. Cytokinin slows the breakdown of chlorophyll.
3. Letham created the assay for excised radish cotyledon expansion. Extracted radish cotyledons are measured and placed in both the test solution and plain water (as a control).
Hence, option 4 is the correct answer.
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