This test is based on the experiments done by Went.
This test is based on the polar transport of auxin in Avena coleoptile.
The auxin applied on one side of the cut coleoptile stump will diffuse down that side only and will cause that side to grow more resulting in curvature of the coleoptiles.
Within limits, this curvature is directly proportional to the amount of auxin applied.
100 curvature is produced by auxin concentration of 150 micrograms per litre at 250C.