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What is the use of mordant in the Gram staining techniques?
Option: 1
Fixation of secondary stain
Option: 2
Fixation of decolouriser
Option: 3
Fixation of primary stain
Option: 4
All of these
Answers (1)
Fixation of primary stain is done by mordant.
A mordant increases the affinity of the cell wall for a stain by binding to the primary stain and thus forming an insoluble complex, which gets trapped in the cell wall.
Iodine solution is used as a mordant in gra staining procedure.
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