With the girdling experiment it has been observed that:
Girdling has no immediate effect on transpiration.
Photosynthates accumulate above the girdle.
The bark below the girdle dies.
All of the above
Girdling experiment demonstrates the translocation of Photosynthates occurs through phloem. In this experiment, the bark of the tree was removed in a ring around the trunk (called girdling). With this experiment it has been observed that girdling has no immediate effect on transpiration. However, transport of Photosynthates in the trunk is blocked at the site where the bark has been removed. Photosynthates accumulate above the girdle. Eventually the bark below the girdle dies, while the bark above swells and remains healthy. Hence, the correct answer is option 4.