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Describe the role of CaCl2 in the preparation of competent cells?

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Divalent ions increase the efficiency of uptake of DNA through the pores in the bacterial cell wall. CaCl2 provides the divalent ion Ca2+ which create transient pores on the bacterial cell wall which facilitate entry of foreign DNA into the bacterial cells. Loading of divalent ions makes the cell competent.

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Gurleen Kaur

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