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  • PCR is a useful tool for early diagnosis of an infectious disease. Elaborate.

PCR is a useful tool for early diagnosis of an infectious disease. Elaborate.

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Use of PCR can amplify the nucleic acids of bacteria or virus. This can help in detecting their presence even when they are in low concentration. This helps in early detection of a disease which was not possible with conventional diagnostic tools. By the time, conventional diagnostic tools could detect a disease; it was very late because of the very high concentration of pathogens. Nevertheless, early detection by using PCR can help in timely and more efficient treatment. Polymerase Chain Reaction (PCR) identifies infections by amplifying specific DNA or RNA from patient samples. If RNA is present, it is first converted to complementary DNA. The procedure includes denaturation (strand separation), annealing (primer binding), and extension (DNA replication) using a thermocycler. The amplified genetic material is then examined to detect the pathogen, allowing for accurate and early diagnosis.

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Gurleen Kaur

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