'Restriction' in Restriction enzyme refers to:
a. Cleaving of the phosphodiester bond in DNA by the enzyme
b. Cutting of DNA at a specific position only
c. Prevention of the multiplication of bacteriophage by the host bacteria
d. All of the above
The answer is the option (c) Prevention of the multiplication of bacteriophage in bacteria
Explanation: Restriction enzymes belong to a class of enzyme called nuclease. Restriction enzymes prevent the multiplication of bacteriophage in bacteria. They are used for cutting of DNA at specific position which becomes possible by cleaving of phosphodiester bond in DNA by the enzyme. Option 'c' is the correct answer.