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Get Answers to all your Questions
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- #Multiple Choice Questions (MCQs)
Significance of 'heat shock' method in bacterial transformation is to facilitate:
a. Binding of DNA to the cell wall
b. Uptake of DNA through membrane transport proteins
c. Uptake of DNA through transient pores in the bacterial cell wall
d. Expression of antibiotic resistance gene
Answers (2)
The answer is the option (c) Uptake of DNA through transient pores in the bacterial cell wall
Explanation: Putting the cells with recombinant DNA on ice; followed by a heat shock and then putting them back on ice helps in uptake of DNA through transient pores in the bacterial cell wall.
The answer is the option (c) Uptake of DNA through transient pores in the bacterial cell wall
Explanation: Putting the cells with recombinant DNA on ice; followed by a heat shock and then putting them back on ice helps in uptake of DNA through transient pores in the bacterial cell wall.
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