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What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?

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Ti plasmid in Agrobacterium has the ability to induce tumours in plants. This plasmid is 'disarmed' by suitable modification, and then it can be used as a cloning vector for delivering gene of interest to plants and animals.

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Gurleen Kaur

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