While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were: Adenine = 29%, Guanine = 17%, Cytosine = 32%, Thymine = 17%. Considering the Chargaff's rule, it can be concluded that:
a. it is a double-stranded circular DNA
b. It is single-stranded DNA
c. It is a double-stranded linear DNA
d. No conclusion can be drawn
The answer is the option (b) It is single-stranded DNA
DNA from any cell of all organisms should have a 1:1 ratio (base pair rule) of pyrimidine and purine bases according to the Chargaff's rules. This implies that the amount of Guanine is equal to cytosine, and the amount of adenine is equal to Thymine. This pattern is common in both strands of the DNA. In this case, the percentage of adenine is not equal to that of Guanine, and the same holds true for cytosine and Thymine. Thus, it is single-stranded DNA.