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  • 'There is a paternity dispute for a child'. Which technique can solve the problem? Discuss the principle involved.

'There is a paternity dispute for a child'. Which technique can solve the problem? Discuss the principle involved.

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DNA fingerprinting can be used to resolve a dispute regarding paternity for a child. DNA fingerprinting is based on the following principle:

DNA fingerprinting: This involves identifying the difference in some specific regions of DNA. The sequence in such regions is called repetitive DNA. A small stretch of DNA is repeated much time in such sequences.

During density, gradient centrifugation, these sequences are separated from bulk DNA as different peaks. The bulk DNA forms major peaks and other small peaks are called satellite DNA. Satellite DNA can be classified into various types, depending on the base composition, length of segment and number of repetitive units. The base composition reveals whether the sequence is A: T rich or G: C rich. These sequences show a high degree of polymorphism and hence form the basis of DNA fingerprinting.

DNA from every tissue shows the same degree of polymorphism in case of an individual. Hence, DNA from any tissue can be utilised to analyse DNA fingerprinting of an individual. Moreover, polymorphism is inheritable from parents to children. Hence. DNA fingerprinting can be utilised to determine the paternity for a child.

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