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The enzyme DNA polymerase in E. coli is a DNA dependent polymerase and also has the ability to proofread the DNA strand being synthesised. Explain. Discuss the dual polymerase.
Answers (1)
DNA polymerase uses a DNA template to catalyse the polymerisation of deoxynucleotides, and hence it is called DNA - dependent. When a new strand of DNA is being processed, this enzyme moves along to increase the speed of polymerisation. While doing so, it "proofreads" the strand being formed. By doing so, it helps in speeding up the process. Hence, its nature can be said as dual, i.e. of reading the template and then proofreading the new strand.
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